The world of embryology is both fascinating and mindblowing, leaving many fertility patients wondering how it all happens. The life-changing work that embryologists do each day make amazing things like IVF possible – and now, they’re answering your top embryology questions!

Skip to a section:

Within the fertility treatment process, embryologists are the people that care for the egg, the sperm, and ultimately, the embryo. They meticulously analyze and watch over the future of someone’s family. As Erica Paganetti puts it, “Embryologists are your child’s first babysitter!”

Erica Paganetti is Illume Fertility’s Senior Embryologist and Embryology Lab Manager. She leads a dedicated team of embryologists, lab technicians and other staff as they foster the early development of embryos – including monitoring, grading, testing, and freezing them.

During the freezing process, also known as cryopreservation, an embryologist is responsible for making sure your embryos, eggs, or sperm are carefully stored for as long as you need.

The field of embryology is full of incredibly complex processes and terminology, which can make it feel, well – a bit mysterious. So, what are embryologists really doing in those dark IVF labs? Is freezing embryos for years and years actually safe? 

Below, Erica answers the top five most frequently asked questions she and her team receive, particularly concerning cryopreservation and embryos created in the IVF process.

Embryos are cultured in our laboratory for a maximum of seven days. For patients who opt to do preimplantation genetic testing (PGT), their embryos are carefully biopsied, then cryopreserved while we wait for results from an outside genetics lab.

Cryopreservation allows us to suspend the development of the embryos until you are ready to use them. The samples collected from each embryo are loaded individually, labeled, and shipped to a genetic testing facility for processing.

So, the short answer is this: We don’t know for sure if the embryos are abnormal or not when we first freeze them, so we have to wait for the genetic testing results to find out.

Genetic testing results have a turnaround time of about two weeks. In the meantime, we freeze all of the embryos in the hopes of normal preimplantation genetic testing (PGT) results.

There can be significant variation in embryo grading, even within a single patient’s group of embryos. We utilize the grade as an indicator of embryo quality, aiding us in selection of the best embryo for procedures such as embryo transfer, cryopreservation, and/or biopsy.

Embryos eligible for these procedures fall within a range of grading criteria specific to our lab. Although there are a range of grades within embryos that can be transferred, cryopreserved, or biopsied, all of these embryos have significant reproductive potential. Note:

Note: Even if you have a lower-graded embryo, it doesn’t always mean that it won’t result in a successful pregnancy and healthy baby! Your Care Team will be able to offer guidance and help answer any questions you have about embryo grading.  

Stories like these are living proof that cryopreserved embryos can be successfully utilized for many, many years after their creation – longer than some ever imagined. 

Cryopreserved semen samples are generally processed and stored in multiple vials for future use. Even if you have multiple vials available for use, when thawing vials of sperm for IUI and/or IVF, vials are warmed and processed one at a time. Additional vials are only thawed when absolutely necessary.

This usually results in additional samples remaining in storage, so please be aware that fees will usually still apply, even after your cycle is complete. Ask your Care Team if you have any questions about your specific storage plan.

Did you learn something new?

We hope so! If you have additional questions about IVF or embryology, send us a message or submit it for consideration in our Ask Monica Q&A video series.

A big thank you to our Senior Embryologist and Lab Manager Erica Paganetti for answering these important questions and providing a little peek inside the incredible world of embryology.